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Summary of Application of Gold Immunochromatography with Gold Label Cassette
The development direction of immunochromatographic analysis is to further improve the sensitivity of detection, to realize multivariate detection and quantitative or semi-quantitative detection.The improvement of sensitivity will undoubtedly broaden the detection range of immunochromatographic analysis, and the use of signal amplification systems or some new markers combined with some corresponding simple detection instruments is one of the more promising ways. For the realization of multiple detection, two methods can be used: multi membrane composite or single membrane multiple receptor fixation, which has great application value for the detection of certain substances with joint detection significance. For quantitative and semi quantitative detection, one approach is to use enzymatic reaction chromatography to quantify by the color development height of the chromatographic strip; Another approach is to quantify the substance to be tested by determining the color development time of the detection band under controlling the amount of sample added.The method is based on the reaction between the content of the sample to be measured and the color development time of the detection band. In addition, the content of the substance to be measured can be determined in a certain concentration range by observing the color development by combining the gold reagent strips with different sensitivities, so as to achieve semi-quantitative detection.
Immunocolloidal gold technology is the fourth largest labeling technology developed after fluorescein, radioisotope, and enzyme labeling technologies. Gold label cassette immunochromatographic strips are the important direction for IVD&POCT. Due to its advantages of simplicity, rapidity, intuitive judgment of results, without special equipment and reagents, It is very suitable for mass detection and large area survey of basic inspection personnel as well as rapid detection of food.
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