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Gold Immunochromatography with Gold label Cassette
Colloidal gold immunochromatography (GICA) is a rapid solid phase membrane immunoassay technology that combines colloidal gold labeling technology, immunoassay technology, and protein chromatography, using nitric acid fiber membranes as carriers. The principle is to use a nitrocellulose membrane as a solid phase carrier, and the sample solution swims on the chromatographic strip by capillary action; At the same time, the substance to be tested in the sample undergoes a highly specific and affinity immune reaction with the receptor (such as antibody or antigen) of the substance to be tested on the chromatography material. During the chromatography process, the immune complex is enriched or intercepted in a certain area (detection band) of the chromatography material, and intuitive experimental results are obtained through the aggregation of directly visible colloidal gold particles within a short period of time (5 to 10 minutes). It does not require the separation of binding markers from free markers, so there are no cumbersome sampling and washing steps as in the Elisa experiment. This technology is characterized by simple and fast operation, clear and intuitive detection results, and requires no special instruments (or only simple instruments), making it particularly suitable for on-site rapid detection. Gold labeled cartridge colloidal gold immunochromatography can be divided into two types according to the molecular size of the substance to be tested: double antibody sandwich method and competitive method. The dual antibody sandwich method is suitable for the detection of macromolecular substances, with strong intuition and high sensitivity, while the competition method is suitable for the detection of small molecular substances.
With the large-scale and intensive development of China's aquaculture industry, the harm of animal infectious diseases to the aquaculture industry is gradually increasing. Therefore, how to rapid diagnostic for effective prevention has become a key link. The appearance of colloidal gold chromatography makes it possible for IVD&POCT.
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